Journal: MethodsX

Article Title: DNA extraction of bacterial cells using a semi-automated filtration system

doi: 10.1016/j.mex.2022.101785

Figure Lengend Snippet: Example of agarose gel picture to illustrate quality of PCR amplified genes from the DNA extracted from the enriched river sample with the various methods. Shown in the gel is the 100 bp DNA ladder (lane 1), Negative control (NTC; lane 2), in-house DNA extraction method, celite 1:420 dil. (Lane3), QIAamp 96 DNA QIAcube HT DNA extraction kit (Qiagen®) (Lane 4), QIAamp 96 DNA QIAcube HT DNA extraction kit (Qiagen®) with proteinase K (Lane 5), adapted in-house method, with binding buffer and proteinase K and no celite (Lane 6), Adapted in-house method, with binding buffer and proteinase K, no celite and no ethanol (Lane 7), adapted in-house method, with binding buffer, no celite, no ethanol and no proteinase K (Lane 8), E. coli DNA ladder (Lane 9), E. coli positive control PCR (Lane 10).

Article Snippet: A commensal non-pathogenic E. coli (ComEC) strain (ATCC 25922) was used in this study for the loading capacity experiment.

Techniques: Agarose Gel Electrophoresis, Amplification, Negative Control, DNA Extraction, Binding Assay, Positive Control

Journal: MethodsX

Article Title: DNA extraction of bacterial cells using a semi-automated filtration system

doi: 10.1016/j.mex.2022.101785

Figure Lengend Snippet:

Article Snippet: A commensal non-pathogenic E. coli (ComEC) strain (ATCC 25922) was used in this study for the loading capacity experiment.

Techniques: DNA Extraction, Purification

Journal: Molecules

Article Title: Commercially Available Viola odorata Oil, Chemical Variability and Antimicrobial Activity

doi: 10.3390/molecules28041676

Figure Lengend Snippet: Antimicrobial activity (mg/mL) of essential oils against the selected pathogen reference strains ( n = 3).

Article Snippet: These included reference strain pathogens related to the infections for which V. odorata oil is most commonly recommended, such as respiratory infections ( Klebsiella pneumoniae ATCC 13883, Streptococcus pyogenes ATCC 12344), skin infections ( Pseudomonas aeruginosa ATCC 27858, Escherichia coli ATCC 25922, Staphylococcus aureus ATCC 25924, Staphylococcus epidermidis ATCC 14990 and Cutibacterium acnes ATCC 6919), opportunistic nosocomial infections ( Acinetobacter baumannii ATCC 17606 and Enterococcus faecium ATCC 8739) and a fungal pathogen reference strain ( Candida albicans ATCC 10231).

Techniques: Activity Assay, Positive Control, Negative Control, Control

Journal: Frontiers in Microbiology

Article Title: Biocontrol of Bacterial Leaf Blight of Rice and Profiling of Secondary Metabolites Produced by Rhizospheric Pseudomonas aeruginosa BRp3

doi: 10.3389/fmicb.2017.01895

Figure Lengend Snippet: Growth promoting and biocontrol determinants of rice rhizosphere associated Pseudomonas aeruginosa .

Article Snippet: Absence of hemolytic activity on blood agar plates (Unpublished data) indicated that rhizospheric P. aeruginosa BRp3 may be unlikely to be a human pathogen (Radhapriya et al., ) but whole genome of this bacterium will be sequenced and compared with non-pathogenic P. aeruginosa strains like ATCC 15442 (Wang et al., ) to better understand the pathogenicity for its safe application.

Techniques: Inhibition

Journal: Frontiers in Microbiology

Article Title: Biocontrol of Bacterial Leaf Blight of Rice and Profiling of Secondary Metabolites Produced by Rhizospheric Pseudomonas aeruginosa BRp3

doi: 10.3389/fmicb.2017.01895

Figure Lengend Snippet: LC/MS chromatogram of Pseudomonas aeruginosa BRp3 extract (24 h growth) demonstrating the presence of (predominantly) 1-hydroxy-phenazine, pyocyanin, and possibly lahorenoic acid analyzed at positive ion mode.

Article Snippet: Absence of hemolytic activity on blood agar plates (Unpublished data) indicated that rhizospheric P. aeruginosa BRp3 may be unlikely to be a human pathogen (Radhapriya et al., ) but whole genome of this bacterium will be sequenced and compared with non-pathogenic P. aeruginosa strains like ATCC 15442 (Wang et al., ) to better understand the pathogenicity for its safe application.

Techniques: Liquid Chromatography with Mass Spectroscopy

Journal: Frontiers in Microbiology

Article Title: Biocontrol of Bacterial Leaf Blight of Rice and Profiling of Secondary Metabolites Produced by Rhizospheric Pseudomonas aeruginosa BRp3

doi: 10.3389/fmicb.2017.01895

Figure Lengend Snippet: Metabolites produced by Pseudomonas aeruginosa BRp3 detected by ESI-MS/MS.

Article Snippet: Absence of hemolytic activity on blood agar plates (Unpublished data) indicated that rhizospheric P. aeruginosa BRp3 may be unlikely to be a human pathogen (Radhapriya et al., ) but whole genome of this bacterium will be sequenced and compared with non-pathogenic P. aeruginosa strains like ATCC 15442 (Wang et al., ) to better understand the pathogenicity for its safe application.

Techniques: Produced

Journal: Frontiers in Microbiology

Article Title: Biocontrol of Bacterial Leaf Blight of Rice and Profiling of Secondary Metabolites Produced by Rhizospheric Pseudomonas aeruginosa BRp3

doi: 10.3389/fmicb.2017.01895

Figure Lengend Snippet: Effects of rice rhizosphere associated Pseudomonas aeruginosa BRp3 for suppression of bacterial leaf blight (BLB) in pot experiments under net house conditions. Antagonistic bacteria i.e., BRp3 was applied both as seed treatment and foliar spray 1 day before clip inoculation of Xoo pathogen. Antibiotic i.e., Streptocyclin @ 5 mg/ pot was sprayed 1 day before clip inoculation (Positive control). Means are an average of four biological replicates and there were 15 plants per replicate. Means followed by the same letter differ non-significantly at p = 0.01 according to DMRT. a Experiment was conducted in small pots and b experiment was conducted in large earthen pots. Different letters show statistical significance of treatments while similar letters show non-significant differences.

Article Snippet: Absence of hemolytic activity on blood agar plates (Unpublished data) indicated that rhizospheric P. aeruginosa BRp3 may be unlikely to be a human pathogen (Radhapriya et al., ) but whole genome of this bacterium will be sequenced and compared with non-pathogenic P. aeruginosa strains like ATCC 15442 (Wang et al., ) to better understand the pathogenicity for its safe application.

Techniques: Positive Control

Journal: Frontiers in Microbiology

Article Title: Biocontrol of Bacterial Leaf Blight of Rice and Profiling of Secondary Metabolites Produced by Rhizospheric Pseudomonas aeruginosa BRp3

doi: 10.3389/fmicb.2017.01895

Figure Lengend Snippet: Induction of defense related enzymes (A) Peroxidase (B) Catalase (C) Polyphenol oxidase (PPO) and (D) Phenylalanine ammonia lyase (PAL) in rice plants inoculated with Pseudomonas aeruginosa BRp3 and challenge inoculated with bacterial leaf blight causing pathogen. Healthy control (HC): Leaves of plants clip inoculated with distilled water, Infected control (IC): Leaves clip inoculated with BLB pathogen i.e., Xanthomonas oryzae pv. oryzae (Xoo). Forty leaves/ treatment were clip inoculated. Bars show standard deviation of four biological replicates and each replicate has 15 plants per replicate.

Article Snippet: Absence of hemolytic activity on blood agar plates (Unpublished data) indicated that rhizospheric P. aeruginosa BRp3 may be unlikely to be a human pathogen (Radhapriya et al., ) but whole genome of this bacterium will be sequenced and compared with non-pathogenic P. aeruginosa strains like ATCC 15442 (Wang et al., ) to better understand the pathogenicity for its safe application.

Techniques: Infection, Standard Deviation

Journal: Frontiers in Microbiology

Article Title: Biocontrol of Bacterial Leaf Blight of Rice and Profiling of Secondary Metabolites Produced by Rhizospheric Pseudomonas aeruginosa BRp3

doi: 10.3389/fmicb.2017.01895

Figure Lengend Snippet: Evaluation of Pseudomonas aeruginosa BRp3 for suppression of bacterial leaf blight (BLB) under field conditions. Healthy control: Leaves of plants clip inoculated with distilled water, Infected control: Leaves clip inoculated with prevalent BLB pathogen i.e., Xanthomonas oryzae pv. o ryzae (Xoo) strain isolated in the present study; Positive control: Leaves clip inoculated with BLB pathogen and plants sprayed with streptocyclin and Pseudomonas sp. BRp3: Leaves clip inoculated with BLB pathogen and plants sprayed with liquid culture of strain BRp3. Sixty leaves/ treatment were clip inoculated. Four plants per replicate were sown in 1 m row. Suppression of BLB was measured in terms of reduction in the mean bacterial blight lesion length on treated leaves compared to those of non-inoculated control. Bars show standard deviation of three biological replicates. Means followed by the same letter differ non-significantly at p = 0.05 according to DMRT.

Article Snippet: Absence of hemolytic activity on blood agar plates (Unpublished data) indicated that rhizospheric P. aeruginosa BRp3 may be unlikely to be a human pathogen (Radhapriya et al., ) but whole genome of this bacterium will be sequenced and compared with non-pathogenic P. aeruginosa strains like ATCC 15442 (Wang et al., ) to better understand the pathogenicity for its safe application.

Techniques: Infection, Isolation, Positive Control, Standard Deviation

Journal: Frontiers in Microbiology

Article Title: Biocontrol of Bacterial Leaf Blight of Rice and Profiling of Secondary Metabolites Produced by Rhizospheric Pseudomonas aeruginosa BRp3

doi: 10.3389/fmicb.2017.01895

Figure Lengend Snippet: Effects of Pseudomonas aeruginosa BRp3 on yield parameters of rice in the presence of Xoo pathogen (A) straw weight and (B) grain weight. Four plants per replicate were sown in 1 m row; Bars show standard deviation of three biological replicates. Means followed by the same letter differ non-significantly at p = 0.05 according to DMRT.

Article Snippet: Absence of hemolytic activity on blood agar plates (Unpublished data) indicated that rhizospheric P. aeruginosa BRp3 may be unlikely to be a human pathogen (Radhapriya et al., ) but whole genome of this bacterium will be sequenced and compared with non-pathogenic P. aeruginosa strains like ATCC 15442 (Wang et al., ) to better understand the pathogenicity for its safe application.

Techniques: Standard Deviation

Journal: Frontiers in Microbiology

Article Title: Biocontrol of Bacterial Leaf Blight of Rice and Profiling of Secondary Metabolites Produced by Rhizospheric Pseudomonas aeruginosa BRp3

doi: 10.3389/fmicb.2017.01895

Figure Lengend Snippet: Effects of Pseudomonas aeruginosa BRp3 on yield parameters of rice variety Super Basmati in a field experiment in the absence of Xoo pathogen. (A) Straw weight and (B) grain weight. Un-inoculated with 80% of the recommended doses of nitrogen (N) and Phosphorus (P) fertilizers. Un-inoculated with full dose of the recommended N and P fertilizers. “ BioPower ” a consortium of bacterial strains is a commercial biofertilizer product of NIBGE. Bars show standard deviation of four biological replicates. Means followed by the same letter differ non-significantly at p = 0.05 according to DMRT. Plot size was 28 m 2 .

Article Snippet: Absence of hemolytic activity on blood agar plates (Unpublished data) indicated that rhizospheric P. aeruginosa BRp3 may be unlikely to be a human pathogen (Radhapriya et al., ) but whole genome of this bacterium will be sequenced and compared with non-pathogenic P. aeruginosa strains like ATCC 15442 (Wang et al., ) to better understand the pathogenicity for its safe application.

Techniques: Standard Deviation

Journal: Frontiers in Microbiology

Article Title: Biocontrol of Bacterial Leaf Blight of Rice and Profiling of Secondary Metabolites Produced by Rhizospheric Pseudomonas aeruginosa BRp3

doi: 10.3389/fmicb.2017.01895

Figure Lengend Snippet: Rice root colonization in response to different treatments in a field experiment. Pseudomonas aeruginosa BRp3 was applied both as seed treatment and foliar spray 1 day before clip inoculation of rice bacterial blight pathogen. Foliar spray of antibiotic streptocyclin was used as positive control while non-inoculated plants with and without pathogen, were used as infected and healthy controls, respectively. Bars represent the standard deviation of three biological replicates. Four plants per replicate were sown in 1 m row and three root samples from each replicate were collected. Means followed by the same letter differ non-significantly at p = 0.05 according to DMRT. CFU, Colony forming units represent total viable count.

Article Snippet: Absence of hemolytic activity on blood agar plates (Unpublished data) indicated that rhizospheric P. aeruginosa BRp3 may be unlikely to be a human pathogen (Radhapriya et al., ) but whole genome of this bacterium will be sequenced and compared with non-pathogenic P. aeruginosa strains like ATCC 15442 (Wang et al., ) to better understand the pathogenicity for its safe application.

Techniques: Positive Control, Infection, Standard Deviation

Journal: Frontiers in Microbiology

Article Title: Biocontrol of Bacterial Leaf Blight of Rice and Profiling of Secondary Metabolites Produced by Rhizospheric Pseudomonas aeruginosa BRp3

doi: 10.3389/fmicb.2017.01895

Figure Lengend Snippet: Survival of Pseudomonas aeruginosa BRp3 on the roots (A) and shoots (B) of field-grown rice plants. Enumeration of bacterial population was determined by viable count method. Root and shoot samples were collected from field-grown rice plants. Four plants per replicate were sown in 1 m row and three root/shoot samples from each replicate were collected. Bars represent the standard deviation. Means followed by the same letter differ non-significantly at p = 0.05 according to DMRT.CFU, Colony forming units.

Article Snippet: Absence of hemolytic activity on blood agar plates (Unpublished data) indicated that rhizospheric P. aeruginosa BRp3 may be unlikely to be a human pathogen (Radhapriya et al., ) but whole genome of this bacterium will be sequenced and compared with non-pathogenic P. aeruginosa strains like ATCC 15442 (Wang et al., ) to better understand the pathogenicity for its safe application.

Techniques: Standard Deviation

Journal: Frontiers in Microbiology

Article Title: Biocontrol of Bacterial Leaf Blight of Rice and Profiling of Secondary Metabolites Produced by Rhizospheric Pseudomonas aeruginosa BRp3

doi: 10.3389/fmicb.2017.01895

Figure Lengend Snippet: Colonization of 21-days old rice roots by Pseudomonas aeruginosa BRp3 studied by fluorescence antibodies staining and CLSM. (A–D) FITC-Immunofluorescence images by confocal laser scanning microscopy (CLSM) of whole root after staining by fluorescent antibody (FA) technique. Un-inoculated control (A) , Rice roots inoculated with Pseudomonas aeruginosa BRp3 (B,C) grown in sterile sand under net house conditions and (D) under field conditions. B, Bacteria; RC, Root cell; RH, Root hair.

Article Snippet: Absence of hemolytic activity on blood agar plates (Unpublished data) indicated that rhizospheric P. aeruginosa BRp3 may be unlikely to be a human pathogen (Radhapriya et al., ) but whole genome of this bacterium will be sequenced and compared with non-pathogenic P. aeruginosa strains like ATCC 15442 (Wang et al., ) to better understand the pathogenicity for its safe application.

Techniques: Fluorescence, Staining, Immunofluorescence, Confocal Laser Scanning Microscopy

Journal: PLoS ONE

Article Title: Recognition of Porphyromonas gingivalis Gingipain Epitopes by Natural IgM Binding to Malondialdehyde Modified Low-Density Lipoprotein

doi: 10.1371/journal.pone.0034910

Figure Lengend Snippet: Binding of anti-MDA-LDL-IgM (MDmAb) and anti-PC-IgM control antibody (α-PC-mAb) to P. gingivalis (Pg) and PC-conjugated bovine serum albumin (PC-BSA) on Western blot (A). Binding of MDmAb to MDA- and MAA-modified and native LDL (nLDL) and PC-BSA using direct binding (B) and competitive (C) chemiluminescence immunoassays. Specific binding of MDmAb to P. gingivalis (Pg) and E. coli was tested with competitive chemiluminescence immunoassay (D). Bacterial suspensions were adjusted to an absorbance of 0.15 at 580 nm with PBS and further diluted as indicated. B/B 0 indicates the ratio of IgM binding with and without a competitor. RLU, relative light unit. Binding of MDmAb and isotype control (cntrl_IgM) to P. gingivalis (E) and E. coli (F) in native conditions was tested with flow cytometry. Fluorescence (FL-1) of the cells with the secondary antibody, +2°Ab (red), MDmAb (blue) and isotype control (green).

Article Snippet: Oral pathogenic bacteria Porphyromonas gingivalis (Pg) strains representing three serotypes, ATCC 33277, W50 and OMGS 434, were used in the study and cultured on Brucella agar plates supplemented with 5% horse blood, 5 μg/ml hemin and 100 mg/ml vitamin K 1 anaerobically for 5 to 6 days.

Techniques: Binding Assay, Control, Western Blot, Modification, Chemiluminescence Immunoassay, Flow Cytometry, Fluorescence

Journal: PLoS ONE

Article Title: Recognition of Porphyromonas gingivalis Gingipain Epitopes by Natural IgM Binding to Malondialdehyde Modified Low-Density Lipoprotein

doi: 10.1371/journal.pone.0034910

Figure Lengend Snippet: A) Schematic presentation of arg-gingipain (RgpA) functional domains cloned and produced in a recombinant system . B) Proteins of P. gingivalis were separated on SDS-PAGE (Pg, gel). Fragments recognized by MDmAb (45, 40 and 32 kDa, black arrows) were identified by mass spectrometry as arginine-specific gingipain or hemagglutinin A of P. gingivalis . , , contain the Mascot results of the database searches and the MSMS spectrum showing the matching amino acids in the peptide sequence. Three domains of the recombinant arg-specific gingipain, RgpCAT, Rgp44 and Rgp15–27 were produced in E. coli and analyzed for recognition by MDmAb and anti-PC-IgM control antibody (α-PC-mAb). C) Specific binding of MDmAb to recombinant gingipain domains Rgp15–27, Rgp44 and RgpCAT was tested with a competitive immunoassay. D) Reciprocally, soluble MDA-LDL, nLDL and PC-BSA were used as competitors for MDmAb binding to Rgp44. B/B 0 indicates the ratio of IgM binding with and without a competitor. MW, molecular weight. PC-BSA, phosphocholine-conjugated bovine serum albumin.

Article Snippet: Oral pathogenic bacteria Porphyromonas gingivalis (Pg) strains representing three serotypes, ATCC 33277, W50 and OMGS 434, were used in the study and cultured on Brucella agar plates supplemented with 5% horse blood, 5 μg/ml hemin and 100 mg/ml vitamin K 1 anaerobically for 5 to 6 days.

Techniques: Functional Assay, Clone Assay, Produced, Recombinant, SDS Page, Mass Spectrometry, Sequencing, Control, Binding Assay, Molecular Weight

Journal: PLoS ONE

Article Title: Recognition of Porphyromonas gingivalis Gingipain Epitopes by Natural IgM Binding to Malondialdehyde Modified Low-Density Lipoprotein

doi: 10.1371/journal.pone.0034910

Figure Lengend Snippet: C57BL/6 mice were immunized with heat-killed P. gingivalis ATCC33277 (Pg; n = 8) and controls received saline (Co; n = 8). Plasma IgM (A) and IgG (B) to MDA-LDL before (pre) and after immunization (post) were determined with chemiluminescence immunoassay. Each C57BL/6 plasma sample (1∶500) was measured in duplicate and an average for each individual was calculated. LDLR −/− mice were immunized with killed P. gingivalis (3 strains mixed) (Pg; n = 7) and controls received PBS (Co; n = 8). Plasma IgM (C) and IgG (D) to MDA-LDL after the second booster immunization (imm) and after the HFD (end) were determined. Each LDLR −/− plasma sample (1∶1000) was measured in duplicate and an average for each individual in two repeated assays was calculated. Additionally, mouse plasma IgM binding to CuOx-LDL (E, G) and PC-BSA (F, H) was determined. For C57BL/6 mice (E, F) this was done similarly as described for panel A. Plasma samples of LDLR −/− mice (G, H) were pooled between three or four mice (1∶1000) for a single assay, in which the mean ± SD within a group is shown.

Article Snippet: Oral pathogenic bacteria Porphyromonas gingivalis (Pg) strains representing three serotypes, ATCC 33277, W50 and OMGS 434, were used in the study and cultured on Brucella agar plates supplemented with 5% horse blood, 5 μg/ml hemin and 100 mg/ml vitamin K 1 anaerobically for 5 to 6 days.

Techniques: Saline, Clinical Proteomics, Chemiluminescence Immunoassay, Binding Assay

Journal: PLoS ONE

Article Title: Recognition of Porphyromonas gingivalis Gingipain Epitopes by Natural IgM Binding to Malondialdehyde Modified Low-Density Lipoprotein

doi: 10.1371/journal.pone.0034910

Figure Lengend Snippet: Plasma antibody levels to P. gingivalis in immunized mice.

Article Snippet: Oral pathogenic bacteria Porphyromonas gingivalis (Pg) strains representing three serotypes, ATCC 33277, W50 and OMGS 434, were used in the study and cultured on Brucella agar plates supplemented with 5% horse blood, 5 μg/ml hemin and 100 mg/ml vitamin K 1 anaerobically for 5 to 6 days.

Techniques: Clinical Proteomics

Journal: PLoS ONE

Article Title: Recognition of Porphyromonas gingivalis Gingipain Epitopes by Natural IgM Binding to Malondialdehyde Modified Low-Density Lipoprotein

doi: 10.1371/journal.pone.0034910

Figure Lengend Snippet: Recombinant proteins of the arginine specific gingipain protease of P. gingivalis were produced in E.coli : two proteins in the hemagglutinin/adhesion domain, Rgp44 and Rgp15–27, and the catalytic domain, RgpCAT, which were used in chemiluminescence immunoassay to determine mouse plasma (1∶500) IgM and IgG binding in P. gingivalis immunized (Pg) and control (Co) groups in both A) C57BL/6 and B) LDLR −/− mice. A) For C57BL/6 immunized and control mice the samples (n = 8 each) were determined as duplicate before (pre) and after (post) immunization. Box-plots represent the distribution of the means of the sample duplicates. B) Two plasma samples within the group were pooled for immunized (black bars) and control (hatched bars) LDLR −/− mice and measured in duplicate. Samples were collected before (pre), after the second booster immunization (imm) and at the end of HFD (end). Columns represent the mean ± SD of pooled samples in each group. **P<0.01 and *P<0.05.

Article Snippet: Oral pathogenic bacteria Porphyromonas gingivalis (Pg) strains representing three serotypes, ATCC 33277, W50 and OMGS 434, were used in the study and cultured on Brucella agar plates supplemented with 5% horse blood, 5 μg/ml hemin and 100 mg/ml vitamin K 1 anaerobically for 5 to 6 days.

Techniques: Recombinant, Produced, Chemiluminescence Immunoassay, Clinical Proteomics, Binding Assay, Control

Journal: PLoS ONE

Article Title: Recognition of Porphyromonas gingivalis Gingipain Epitopes by Natural IgM Binding to Malondialdehyde Modified Low-Density Lipoprotein

doi: 10.1371/journal.pone.0034910

Figure Lengend Snippet: A, B) C57BL/6 mice were immunized with heat-killed P. gingivalis ATCC33277 (Pg) and controls (Co) received saline. The plasma was diluted 1∶100–1∶6400 and IgM binding to MDA-LDL, CuOx-LDL, native LDL, PC-BSA (A), P. gingivalis and recombinant gingipain domains Rgp44, Rgp15–27 and RgpCAT (B) were determined before (pre) and after (post) immunization with chemiluminescence immunoassay. Mean ± SD for two samples is shown. C, D) LDLR −/− mice were immunized with killed P. gingivalis (3 strains mixed) (Pg) and controls (Co) received PBS. Pooled plasma from two mice was used for each dilution curve, and mean ± SD for two dilution curves is shown. IgM binding to MDA-LDL, CuOx-LDL, native LDL, PC-BSA (C), P. gingivalis and recombinant gingipain domain Rgp44, Rgp15–27 and RgpCAT (D) were determined before (pre) and after the second booster immunization (imm) as described.

Article Snippet: Oral pathogenic bacteria Porphyromonas gingivalis (Pg) strains representing three serotypes, ATCC 33277, W50 and OMGS 434, were used in the study and cultured on Brucella agar plates supplemented with 5% horse blood, 5 μg/ml hemin and 100 mg/ml vitamin K 1 anaerobically for 5 to 6 days.

Techniques: Saline, Clinical Proteomics, Binding Assay, Recombinant, Chemiluminescence Immunoassay

Journal: PLoS ONE

Article Title: Recognition of Porphyromonas gingivalis Gingipain Epitopes by Natural IgM Binding to Malondialdehyde Modified Low-Density Lipoprotein

doi: 10.1371/journal.pone.0034910

Figure Lengend Snippet: LDLR −/− mice (n = 7) were immunized without adjuvant with killed P. gingivalis (3 strains mixed) (Pg) followed by high fat diet (HFD). Controls (PBS, n = 8) received PBS. A) The extent of atherosclerotic plaque development was determined after HFD by en face analysis of the Sudan IV -stained aortas. B) Lesions at the aortic origin were measured on histological sections as percentage of plaque area in the aorta cross-sectional area. Representative pictures of aortas and cross-sections are shown for each group. * P<0.05.

Article Snippet: Oral pathogenic bacteria Porphyromonas gingivalis (Pg) strains representing three serotypes, ATCC 33277, W50 and OMGS 434, were used in the study and cultured on Brucella agar plates supplemented with 5% horse blood, 5 μg/ml hemin and 100 mg/ml vitamin K 1 anaerobically for 5 to 6 days.

Techniques: Adjuvant, Staining

Journal: AMB Express

Article Title: Evaluation of the antibacterial potential of silver nanoparticles synthesized through the interaction of antibiotic and aqueous callus extract of Fagonia indica

doi: 10.1186/s13568-019-0797-2

Figure Lengend Snippet: Average zones of inhibition of different bacteria grown in the presence of callus extract mediated AgNPs, ciprofloxacin + callus extract medicated AgNPs and ciprofloxacin only as control

Article Snippet: The antibacterial activity of AgNPs was carried out against four pathogenic strains Escherichia coli (ATCC 23716), Citrobacter amalonaticus (ATCC 25405) , Shigella sonnei (ATCC 29930) , and Salmonella typhi (ATCC 35664).

Techniques: Inhibition, Bacteria, Control

Journal: AMB Express

Article Title: Evaluation of the antibacterial potential of silver nanoparticles synthesized through the interaction of antibiotic and aqueous callus extract of Fagonia indica

doi: 10.1186/s13568-019-0797-2

Figure Lengend Snippet: Activity of ciprofloxacin + callus extract mediated AgNPs, callus extract mediated AgNPs, ciprofloxacin only and extract only controls against Escherichia coli

Article Snippet: The antibacterial activity of AgNPs was carried out against four pathogenic strains Escherichia coli (ATCC 23716), Citrobacter amalonaticus (ATCC 25405) , Shigella sonnei (ATCC 29930) , and Salmonella typhi (ATCC 35664).

Techniques: Activity Assay

Journal: BMC Microbiology

Article Title: When more is less: Emergent suppressive interactions in three-drug combinations

doi: 10.1186/s12866-017-1017-3

Figure Lengend Snippet: Comparison of suppressive interactions in pairwise versus emergent three-drug interactions. Bars represent the proportion of interactions that are classified as antagonistic suppressive in pairwise interactions (black) and in three-drug interactions (white) for three bacteria strains: wild-type, non-pathogenic E. coli BW25113; pathogenic E. coli CFT073; and non-pathogenic S. epidermidis 14990. For the data presented in this paper, 95% confidence intervals resulting from bootstrapping experiments are shown

Article Snippet: Additionally, drug combinations were also tested in (1) the pathogenic E. coli strain CFT073, a highly virulent pyelonephritis strain isolated from human clinical specimen (from ATCC) and (2) Staphylococcus epidermidis 14990 (from ATCC).

Techniques: Comparison, Bacteria

Journal: BMC Microbiology

Article Title: When more is less: Emergent suppressive interactions in three-drug combinations

doi: 10.1186/s12866-017-1017-3

Figure Lengend Snippet: Suppressor and suppressee antibiotics. For each antibiotic, the number of suppressive interactions in E. coli BW25113 in which it acts as the suppressor (x axis) and the suppressee (y axis) are plotted. Antibiotic abbreviations are as listed in Table

Article Snippet: Additionally, drug combinations were also tested in (1) the pathogenic E. coli strain CFT073, a highly virulent pyelonephritis strain isolated from human clinical specimen (from ATCC) and (2) Staphylococcus epidermidis 14990 (from ATCC).

Techniques:

Journal: BMC Microbiology

Article Title: When more is less: Emergent suppressive interactions in three-drug combinations

doi: 10.1186/s12866-017-1017-3

Figure Lengend Snippet: Suppressive three-drug interactions in E. coli BW25113. Growth measurements are shown for bacteria in single-drug, two-drug, and three-drug conditions relative to the no-drug control (100% growth, not shown). Emergent suppression was determined following Tekin et al. (see ). The upper left figure is a schematic with X, Y, and Z representing three different drugs. Only the experimental data for the three-drug combination (X + Y + Z), the suppressed pairwise drug combination with the lowest growth (Y + Z), and the suppressor single drug (X) is shown. 46 triple combinations of a total 327 interaction measures were determined to be suppressive. Antibiotic abbreviations are as listed in Table

Article Snippet: Additionally, drug combinations were also tested in (1) the pathogenic E. coli strain CFT073, a highly virulent pyelonephritis strain isolated from human clinical specimen (from ATCC) and (2) Staphylococcus epidermidis 14990 (from ATCC).

Techniques: Bacteria, Control